Incorporation of [2,3,4,5,6-2H5]Phenylalanine, [3,5-2H2]Tyrosine, and [2,4,5,6,7-2H5]Tryptophan into... - статья по английскому языку

 

Тезисы:

  • Phenylalanine added into the growth medium.
  • H-labeled aromatic amino acids into the protein molecule.
  • The protein must be transformed into the soluble form by solubilization in 0.5% SDS.
  • Delipidation and precipitation of the protein were combined into the same stage.
  • Low yield of BR was no barrier to further studies of isotopic incorporation.
  • H-labeled amino acids into the BR molecule was of a specific type.
  • 91 for control; data not shown) , confirm the presence of deuterium in phenylalanine.
  • 426, 8%, control) represents the additional methylation at a-amino group of phenylalanine.
  • H-labeled aromatic amino acids into the BR molecule.
  • Phenylalanine isolated by reverse-phase HPLC.
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